Each digital PCR platform has its own hands-on protocol. In this item, we have chosen to describe protocols used on…

Copy Number Variation (CNV) detection is a critical tool for estimating the variation in the copy number of genomic segments,…

In order to ensure experimental transparency, consistency between research laboratories and thus, maintain a high level of integrity in publications,…

Poisson distribution, named after the French mathematician Siméon Denis Poisson, is the probability of occurrence of a given number of events in…

First of all, let’s assume that a target gene concentration has been estimated together with its relative uncertainty, by applying…

When the different populations are not well separated, automatic thresholding fails and must be manually adjusted. The threshold is usually set…

DNA preparation is a crucial step for the success of your digital PCR assay. Template extraction, its quality control, and…

Sub-optimal digital PCR settings very often lead to insufficient signal to noise ratio. This affects the separation between the negative…

Primer and probe design is a crucial step for a successful experiment. The rules for designing primers and probes in…