How To Setup Analyze and Report for PCR Primer Design
Digital PCR Tutorials
Performing Digital PCR Hands-On
Each digital PCR platform has its own hands-on protocol. In this item, we have chosen to describe protocols used on…
Copy Number Variation Calculation for your PCR Experiment
Copy Number Variation (CNV) detection is a critical tool for estimating the variation in the copy number of genomic segments,…
MIQE Guidelines
In order to ensure experimental transparency, consistency between research laboratories and thus, maintain a high level of integrity in publications,…
Poisson Law Computation
Poisson distribution, named after the French mathematician Siméon Denis Poisson, is the probability of occurrence of a given number of events in…
Dynamic Ranges of Detection & Quantification
First of all, let’s assume that a target gene concentration has been estimated together with its relative uncertainty, by applying…
Advanced Threshold Setting
When the different populations are not well separated, automatic thresholding fails and must be manually adjusted. The threshold is usually set…
DNA Preparation for Digital PCR
DNA preparation is a crucial step for the success of your digital PCR assay. Template extraction, its quality control, and…
Digital PCR Assay Optimization
Sub-optimal digital PCR settings very often lead to insufficient signal to noise ratio. This affects the separation between the negative…
Designing Primers & Probes
Primer and probe design is a crucial step for a successful experiment. The rules for designing primers and probes in…