Digital PCR Reagents and Chips Compatible with the Nio™ and Naica® System
Digital PCR workflows do not need to be challenging. Stilla Technologies cutting-edge patented microfluidic innovation integrates the Crystal Digital PCR® workflow into a single consumable. Combined with specially-formulated Nio™ and naica® PCR MIX reagents, researchers get maximized sensitivity and flexible throughput with simple reaction assembly and minimal hands-on time.
Crystal Digital PCR® Chip Consumables
Stilla offers two types of easy-to-use Crystal Digital PCR® chip consumables. Depending on your individual digital PCR workflow needs, select the optimal chip type for your application. After sample preparation, each chip requires just a single pipetting step before being loaded into the Nio+ or naica® system for partitioning, PCR amplification, and sample read-out.
Ruby Chip
For higher throughput applications, the Ruby Chip offers the analysis of up to 384 samples per run. The Ruby Chip is also compatible with integration to automated liquid handling equipment. Made for the Nio™ and naica® systems.
| Capacity | 16 samples/chip |
| Input volume | 5 µL/chamber |
| Droplets per sample | Up to 17,000 |
| Dynamic range* | ~ 4.7 log |
| Compatible systems | Nio™ and naica® |
Sapphire Chip
For maximum template input volume and high sensitivity applications, the Sapphire Chip can be loaded with up to 4 samples per chip, and generate up to 30,000 droplets per sample.
The Sapphire Chip supports applications such as rare mutation detection, liquid biopsy, gene expression analysis, and pathogen detection. Made for the naica® systems.
| Capacity | Up to 4 samples/chip |
| Input volume | 25 µL/chamber |
| Droplets per sample | Up to 30,000 |
| Dynamic range* | ~ 5 log |
| Compatible systems | naica® |
Crystal Digital PCR® Reagents
Specially-formulated Nio™ and naica® PCR reagents ensure optimal performance for Crystal Digital PCR®. Through Stilla’s ready-to-use reagents solutions, acquire sensitive measurements for diverse DNA and RNA applications on the Nio™ and naica® system.
Crystal Universal Reporters
Crystal Universal Reporters generate fluorescent signals when activated during a Crystal Digital PCR® reaction using target-specific Crystal Flex Probe detection chemistry.
Their universal design allows them to be used with any Crystal Digital PCR® Assay, providing a modular approach to detection. This means you can use the same set of Crystal Universal Reporters to detect and quantify all targets of interest, regardless of the specific assay or the degree of multiplexing.
Select the Crystal Universal Reporter compatible with the Crystal Digital PCR® Assay of interest, either in a 200 or a 1000 Ruby Chip reaction format.
| Reference | Product Name | Volume |
|---|---|---|
| R41401 | Crystal Universal Reporter 3 | 30 µL |
| R41402 | Crystal Universal Reporter 3 | 150 µL |
| R42401 | Crystal Universal Reporter 7 | 30 µL |
| R42402 | Crystal Universal Reporter 7 | 150 µL |
Crystal Universal Reporters technical resources
naica® multiplex PCR MIX (TaqMan® compatible)
The naica® multiplex PCR MIX is a ready-to-use two-component solution specially formulated for optimal TaqMan® fluorescent probe-based Crystal Digital PCR® and high multiplexing capacity.
-The stably incorporated internal blue channel reference dye facilitates reaction assembly while ensuring reliable partition detection.
-The unique 10X concentration liberates a reaction volume that can instead be utilized by additional sample input, probes, or primers, which increases multiplexing capacity and provides superior detection sensitivity for low concentration samples and rare targets.
| Reference | Product Name | Volume |
|---|---|---|
| R10054 | naica® multiplex PCR MIX 5X | 0.750mL |
| R10055 | naica® multiplex PCR MIX 5X | 1.50mL |
| R10104 | naica® multiplex PCR MIX 10X | 0.375mL |
| R10105 | naica® multiplex PCR MIX 10X | 0.750mL |
naica® multiplex PCR MIX technical resources
naica® PCR MIX (Crystal Flex Probe and EvaGreen® compatible)
The naica® PCR MIX is a ready-to-use two-component solution optimized for Crystal Flex Probe technology and EvaGreen® fluorescent intercalating dye-based Crystal Digital PCR®.
| Reference | Product Name | Volume |
|---|---|---|
| R10056 | naica® PCR MIX 5X | 1.50mL |
| R10106 | naica® PCR MIX 10X | 0.750mL |
naica® PCR MIX technical resources
naica® IQ/OQ Kit
Routine Operational Qualification (OQ) procedures of the naica® system and Nio™+ can be performed using the naica® IQ/OQ Kit, a ready-to-use OQ solution to closely monitor equipment compliance to operating specifications. Use the naica® IQ/OQ Kit to qualify the naica® system and Nio™+ on a periodic basis in accordance with established standard operating procedures (SOPs).
Experience the streamlined routine OQ solution with the naica® IQ/OQ Kit for a wide range of key nucleic acid detection and quantification applications, validated for use on all Stilla Technologies dPCR platforms.
| Reference | Product Name | Number of Reactions |
|---|---|---|
| R30001 | naica® IQ/OQ Kit | 24 reactions on the Sapphire Chip (2 full OQ of the naica® system) 96 reactions on the Ruby Chip (1 full OQ of the Nio™+) |
naica® IQ/OQ Kit technical resources
Related Products
For Research Use Only. Not for use in diagnostic procedures.
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