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Digital PCR Reagents and Chips Compatible with the Naica® System

Digital PCR workflows do not need to be challenging. Stilla Technologies cutting-edge patented microfluidic innovation integrates the Crystal Digital PCR™ workflow into a single consumable. Combined with specially-formulated naica® PCR MIX reagents, researchers get maximized sensitivity and flexible throughput with simple reaction assembly and minimal hands-on time.

Crystal Digital PCR™ Chip Consumables

Stilla offers two types of easy-to-use Crystal Digital PCR™ chip consumables. Depending on your individual digital PCR workflow needs, select the optimal chip type for your application. After sample preparation, each chip requires just a single pipetting step before being loaded into the naica® system for partitioning, PCR amplification, and sample read-out.

Ruby Chip

For higher throughput applications, the Ruby Chip offers the analysis of up to 48 samples per run. The Ruby Chip is also compatible with integration to automated liquid handling equipment.

Capacity  16 samples/chip 
Input volume  5 µL/chamber 
Droplets per sample  Up to 17,000  
Dynamic range*  ~ 4.7 log 
*analytical specifications are assay-dependent

Sapphire Chip

For maximum template input volume and high sensitivity applications, the Sapphire Chip can be loaded with up to 4 samples per chip, and generate up to 30,000 droplets per sample.
The Sapphire Chip supports applications such as rare mutation detection, liquid biopsy, gene expression analysis, and pathogen detection.

Capacity  Up to 4 samples/chip 
Input volume  25 µL/chamber 
Droplets per sample  Up to 30,000
Dynamic range*  ~ 5 log 
*analytical specifications are assay-dependent

Crystal Digital PCR™ Reagents

Specially-formulated naica® PCR MIX reagents ensure optimal performance for Crystal Digital PCR™.

naica® multiplex PCR MIX (TaqMan® compatible)

The naica® multiplex PCR MIX is a ready-to-use two-component solution specially formulated for optimal TaqMan® fluorescent probe-based Crystal Digital PCR™ and high multiplexing capacity.

  • The stably incorporated internal blue channel reference dye facilitates reaction assembly while ensuring reliable partition detection.
  • The unique 10X concentration liberates a reaction volume that can instead be utilized by additional sample input, probes, or primers, which increases multiplexing capacity and provides superior detection sensitivity for low concentration samples and rare targets.
ReferenceProduct NameVolume
R10054naica® multiplex PCR MIX 5X0.750mL
R10055naica® multiplex PCR MIX 5X1.50mL
R10104naica® multiplex PCR MIX 10X0.375mL
R10105naica® multiplex PCR MIX 10X0.750mL

naica® multiplex PCR MIX technical resources

  • Combined Shape

    Instructions for Use

  • Combined Shape

    MSDS

  • Combined Shape

    Sensitive & Flexible Crystal Digital PCR quantification using the naica® multiplex PCR MIX

  • Combined Shape

    Robust and sensitive 6-color Crystal Digital PCR using the naica® multiplex PCR MIX

  • naica® PCR MIX (EvaGreen® compatible)

    The naica® PCR MIX is a ready-to-use two-component solution optimized for EvaGreen® fluorescent intercalating dye-based Crystal Digital PCR™.

    ReferenceProduct NameVolume
    R10056naica® PCR MIX 5X1.50mL
    R10106naica® PCR MIX 10X0.750mL

    naica® PCR MIX technical resources

  • Combined Shape

    Instructions for Use

  • Combined Shape

    MSDS

  • Combined Shape

    Accurate Crystal Digital PCR™ quantification of a human target gene using the maica PCR MIX and EvaGreen

  • Related Products

    For Research Use Only. Not for use in diagnostic procedures.

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