Digital PCR Reagents and Chips Compatible with the Naica® System
Digital PCR workflows do not need to be challenging. Stilla Technologies cutting-edge patented microfluidic innovation integrates the Crystal Digital PCR™ workflow into a single consumable. Combined with specially-formulated naica® PCR MIX reagents, researchers get maximized sensitivity and flexible throughput with simple reaction assembly and minimal hands-on time.
Crystal Digital PCR™ Chip Consumables
Stilla offers two types of easy-to-use Crystal Digital PCR™ chip consumables. Depending on your individual digital PCR workflow needs, select the optimal chip type for your application. After sample preparation, each chip requires just a single pipetting step before being loaded into the naica® system for partitioning, PCR amplification, and sample read-out.
Ruby Chip
For higher throughput applications, the Ruby Chip offers the analysis of up to 48 samples per run. The Ruby Chip is also compatible with integration to automated liquid handling equipment.
| Capacity | 16 samples/chip |
| Input volume | 5 µL/chamber |
| Droplets per sample | Up to 17,000 |
| Dynamic range* | ~ 4.7 log |
Sapphire Chip
For maximum template input volume and high sensitivity applications, the Sapphire Chip can be loaded with up to 4 samples per chip, and generate up to 30,000 droplets per sample.
The Sapphire Chip supports applications such as rare mutation detection, liquid biopsy, gene expression analysis, and pathogen detection.
| Capacity | Up to 4 samples/chip |
| Input volume | 25 µL/chamber |
| Droplets per sample | Up to 30,000 |
| Dynamic range* | ~ 5 log |
Crystal Digital PCR™ Reagents
Specially-formulated naica® PCR reagents ensure optimal performance for Crystal Digital PCR™ on the naica® system.
naica® multiplex PCR MIX (TaqMan® compatible)
The naica® multiplex PCR MIX is a ready-to-use two-component solution specially formulated for optimal TaqMan® fluorescent probe-based Crystal Digital PCR™ and high multiplexing capacity.
-The stably incorporated internal blue channel reference dye facilitates reaction assembly while ensuring reliable partition detection.
-The unique 10X concentration liberates a reaction volume that can instead be utilized by additional sample input, probes, or primers, which increases multiplexing capacity and provides superior detection sensitivity for low concentration samples and rare targets.
| Reference | Product Name | Volume |
|---|---|---|
| R10054 | naica® multiplex PCR MIX 5X | 0.750mL |
| R10055 | naica® multiplex PCR MIX 5X | 1.50mL |
| R10104 | naica® multiplex PCR MIX 10X | 0.375mL |
| R10105 | naica® multiplex PCR MIX 10X | 0.750mL |
naica® multiplex PCR MIX technical resources
naica® PCR MIX (EvaGreen® compatible)
The naica® PCR MIX is a ready-to-use two-component solution optimized for EvaGreen® fluorescent intercalating dye-based Crystal Digital PCR™.
| Reference | Product Name | Volume |
|---|---|---|
| R10056 | naica® PCR MIX 5X | 1.50mL |
| R10106 | naica® PCR MIX 10X | 0.750mL |
naica® PCR MIX technical resources
naica® IQ/OQ Kit
Routine Operational Qualification (OQ) procedures of the naica® system can be performed using the naica® IQ/OQ Kit, a ready-to-use OQ solution to closely monitor equipment compliance to operating specifications. Use the naica® IQ/OQ Kit to qualify the naica® system on a periodic basis in accordance with established standard operating procedures (SOPs).
Experience the streamlined routine OQ solution with the naica® IQ/OQ Kit for a wide range of key nucleic acid detection and quantification applications, validated for use on both the 3-color and the 6-color naica® system.
| Reference | Product Name | Number of Reactions |
|---|---|---|
| R30001 | naica® IQ/OQ Kit | 24 reactions on the Sapphire Chip (2 full OQ of the naica® system) |
naica® IQ/OQ Kit technical resources
Related Products
For Research Use Only. Not for use in diagnostic procedures.
Request a Quote
Harness the power of the naica® system and get started with Crystal Digital PCR™ today.