Digital PCR Reagents and Chips Compatible with the Naica® System
Digital PCR workflows do not need to be challenging. Stilla Technologies cutting-edge patented microfluidic innovation integrates the Crystal Digital PCR™ workflow into a single consumable. Combined with specially-formulated naica® PCR MIX reagents, researchers get maximized sensitivity and flexible throughput with simple reaction assembly and minimal hands-on time.
Crystal Digital PCR™ Chip Consumables
Stilla offers two types of easy-to-use Crystal Digital PCR™ chip consumables. Depending on your individual digital PCR workflow needs, select the optimal chip type for your application. After sample preparation, each chip requires just a single pipetting step before being loaded into the naica® system for partitioning, PCR amplification, and sample read-out.
For higher throughput applications, the Ruby Chip offers the analysis of up to 48 samples per run. The Ruby Chip is also compatible with integration to automated liquid handling equipment.
|Input volume||5 µL/chamber|
|Droplets per sample||Up to 17,000|
|Dynamic range*||~ 4.7 log|
For maximum template input volume and high sensitivity applications, the Sapphire Chip can be loaded with up to 4 samples per chip, and generate up to 30,000 droplets per sample.
The Sapphire Chip supports applications such as rare mutation detection, liquid biopsy, gene expression analysis, and pathogen detection.
|Capacity||Up to 4 samples/chip|
|Input volume||25 µL/chamber|
|Droplets per sample||Up to 30,000|
|Dynamic range*||~ 5 log|
Crystal Digital PCR™ Reagents
Specially-formulated naica® PCR MIX reagents ensure optimal performance for Crystal Digital PCR™.
naica® multiplex PCR MIX (TaqMan® compatible)
The naica® multiplex PCR MIX is a ready-to-use two-component solution specially formulated for optimal TaqMan® fluorescent probe-based Crystal Digital PCR™ and high multiplexing capacity.
- The stably incorporated internal blue channel reference dye facilitates reaction assembly while ensuring reliable partition detection.
- The unique 10X concentration liberates a reaction volume that can instead be utilized by additional sample input, probes, or primers, which increases multiplexing capacity and provides superior detection sensitivity for low concentration samples and rare targets.
|R10054||naica® multiplex PCR MIX 5X||0.750mL|
|R10055||naica® multiplex PCR MIX 5X||1.50mL|
|R10104||naica® multiplex PCR MIX 10X||0.375mL|
|R10105||naica® multiplex PCR MIX 10X||0.750mL|
naica® multiplex PCR MIX technical resources
Instructions for Use
Sensitive & Flexible Crystal Digital PCR quantification using the naica® multiplex PCR MIX
Robust and sensitive 6-color Crystal Digital PCR using the naica® multiplex PCR MIX
naica® PCR MIX (EvaGreen® compatible)
The naica® PCR MIX is a ready-to-use two-component solution optimized for EvaGreen® fluorescent intercalating dye-based Crystal Digital PCR™.
|R10056||naica® PCR MIX 5X||1.50mL|
|R10106||naica® PCR MIX 10X||0.750mL|
naica® PCR MIX technical resources
Instructions for Use
Accurate Crystal Digital PCR™ quantification of a human target gene using the maica PCR MIX and EvaGreen
For Research Use Only. Not for use in diagnostic procedures.
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