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Organ transplant testing and rare event detection

Digital PCR for rare event detection

Donor-derived cell-free DNA (dd-cfDNA) is gaining adoption as a biomarker for monitoring solid organ transplants. Using a minimally-invasive liquid biopsy approach, as opposed to a more invasive tissue biopsy,  transplant recipients can be monitored for potential cases of rejection. Using this approach of identifying and quantifying cfDNA in patients can be more cost-effective than traditional approaches and  affords earlier detection and management of rejection.

Sequencing techniques, while thorough, can be costly and yield high turnaround times, while qPCR methods might not detect low-levels of dd-cfDNA. Digital PCR provides better quantification of cfDNA – even at a lower levels – while also providing faster turnaround times, lower costs, and better sample control than sequencing.

From sample extraction to analysis for transplant diagnostics

The naica® system offers a fast, easy-to-use platform for quantification of genetic targets. In order to detect and quantify multiple genetic targets in a single run, the naica® system also offers the highest multiplexing available on the market today. When coupled with a sample preparation platform such as the Maxwell® RSC instruments from Promega, users are provided with a seamless end-to-end workflow – from sample preparation to analysis.

Digital PCR for rare event detection

Digital PCR for rare event detection

Stilla provides digital PCR kits out-of-the-box, as well as custom assay design services in order to help researchers characterize and quantify genetic targets for applications detecting and quantifying rare events for transplant diagnostics.

  1. Application Notes

    Accurate monitoring of chimerism after hematopoietic stem cell transplantation using Crystal Digital PCR™

  2. Webinar

    Crystal Digital PCR™ for accurate chimerism monitoring after stem cell transplantation

  3. Poster

    The 6-color naica® system: A digital PCR platform and workflow for high multiplex genetic detection and analysis

  4. Poster

     A streamlined workflow for liquid biopsy sample extraction and highplex digital PCR analysis using the Maxwell® CSC system and 6-color Crystal Digital PCR™

  5. Publications

    Digital Polymerase Chain Reaction for Assessment of Mutant Mitochondrial Carry-over after Nuclear Transfer for In Vitro Fertilization

  6. Webinar

     Advancing high-plex digital PCR into the mainstream


Getting started with digital PCR

From custom assay design services to onsite training, Stilla has the knowledge and expertise to help researchers get started on their workflow. Stilla technologies has compiled several statistical tools to help get your started with your digital PCR assays, including:

For Research Use Only. Not for use in diagnostic procedures.

Learn more about what the naica® system and Crystal Digital PCR™ can do for your research