The Next Generation Droplet Digital PCR.
Nio® Digital PCR offers a simple digital PCR workflow that supports 7 colors, a capacity of up to 768 samples per 8-hour workday, continuous loading, and easy-to-use software.
Nio® in 3 minutes
The next leap forward in digital PCR.
Learn more in our latest application & technical notes.
Digital PCR can be used for highly accurate DNA quantification. Read our latest application & technical notes:
- ESR1 mutations
- EGFR mutations
- CRISPR QC
ESR1 mutation detection in FFPE and Plasma DNA of breast cancer samples using Crystal Digital PCR® and Nio®+.
To detect and quantify the most frequent ESR1 mutations in a single test, this application note explores the ESR1 12-plex dPCR assay. FFPE DNA and Plasma cfDNA samples from breast cancer patients were analyzed using this assay on the Nio®+ system and the results were compared to previously obtained cdPCR results from Centre Eugène Marquis.
EGFR mutation detection in NSCLC FFPE samples using Crystal Digital PCR® and Nio®+
In this application note, we describe an assay to detect and quantify several EGFR mutations in a single test, without sacrificing precision or reliability of the results, using the EGFR 6-color Crystal Digital PCR® Kit . The FFPE DNA NSCLC samples were analyzed using this 6-color multiplex assay on Nio®+ and the results were compared to previous NGS results obtained. This study was performed at the CHU Pontchaillou (Rennes, France).
On- and off-target detection in a CRISPR-edited mammalian cell line using a single-step 3-color Crystal Digital PCR™ assay
The generation and usage of homozygous fluorescent knock-in mammalian cell lines using sequence-specific Cas-endonucleases is a commonly used procedure. However, to identify the desired genetic modification and reject clones with off-target events, hundreds of monoclonal colonies need to be screened. The maintenance and screening of these clones is a laborious, time-consuming, and error-prone process. With the European Molecular Biology Laboratory, we implemented a single-step 3-color assay using Crystal Digital PCR™ to assess on-target copy number and detect off-target events simultaneously.
Reagents & consumables.
Everything you need to get started with digital PCR.
Trusted by the world’s leading scientists.
Customer testimonials
They – Stilla Technologies – have something that I really appreciate, which is a very close contact with the client.
This, for me, is one of the biggest advantages. We don’t know everything, and having the potential to share with the company our doubts, our experiences, and our expectations is something very important.
Zelia Gouveia, PhD
Head of the Process
Development of cell-based therapies | CellAction
Customer testimonials
Nio® workflow is really simple. You need to develop a Nio® protocol and a Nio® experiment, combine them with the software, prepare the ruby chip, and feed in the Nio® device, and it’s done.
Adrien Savy, PhD
AAV Principal Scientist | Coave Therapeutics
Customer testimonials
The user interface of the Nio®+ impressed me the most. It is very easy to operate. You have everything nicely grouped up.
Bern de Lignie
R&D Scientist | Zoolyx
Customer testimonials
Using the Nio®+ we experienced peace of mind. Thanks to the continuous loading you never need to worry about the availability of the machine. It’s a streamlined and simplified workflow. Stilla Technologies is a driving force for innovation!
Dr. Alexandra Lespagnol
Biological Scientist | CHU de Rennes
Customer testimonials
The Nio®+ workflow is a breeze. The software is very intuitive. It’s a machine that works together with you and offers the flexibility for multiple users to run experiments on the same day. We’re always amazed by the high-level of knowledge from the Stilla team on digital PCR.
Wim Trypsteen, PhD
Postdoctoral Researcher | Ghent University
Co-Coordinator | Digital PCR Consortium
Customer testimonials
The main difference that we experience from the workflow with droplet digital PCR® to the Nio®+ workflow is the transferring of plates from one machine to the other. If you have four machines instead of one, of course you have to move from one machine to the other.
Antonio Salgado-Somoza, PhD
Senior Analytical Scientist | Exothera
Customer testimonials
It’s a compact machine, fully integrated with an easy-to-use interface. For me, it’s the best.
Elise Devos, PhD
Sr. Scientist Molecular Diagnostics | BioMerieux
Kits and assays.
Get started with our kits and assays.
EGFR 6-color Crystal Digital PCR™ Kit
The EGFR 6-color Crystal Digital PCR™ kit reliably detects 32 common, rare, activating, and resistant somatic EGFR mutations in a single assay.
naica® IQ/OQ Kit
A ready-to-use OQ solution to closely monitor equipment compliance to operating specifications.
KRAS (drop-off G12-G13) Crystal Digital PCR® Assay
Assay-specific Crystal Flex Probes for the detection of KRAS mutations and its Positive Control.
GSTP1 Methylation Crystal Digital PCR® Assay
Assay-specific Crystal Flex Probes for the detection of GSTP1 Methylation and its Positive Control.
ESR1 (17 mutations) Crystal Digital PCR® Assay
Assay-specific Crystal Flex Probes for the detection of ESR1 mutations and its Positive Control.
Your custom assays
In silico design services are offered, encompassing everything from design to wet lab testing.
What can I do with digital PCR?
Discover our applications to see how you can get started with dPCR.
For liquid biopsy & oncology
For cell & gene therapy
For environmental & food testing
For pathogen detection
For organ transplant testing and rare event detection
Brand new: Meet Nio®.
For those managing hundreds of samples, or those managing highly variable sample inflow. Or those in between.
7-colors
Nio® is the only 7-color instrument on the market, giving you easy access to 7 target capabilities. For even higher plexed assays, you can combine colors to achieve well over 20 targets in a single chamber; natively supported in our software.
384 chambers per day
In any 8 hour span of laboratory work, Nio®+ offers a capacity of 384 chambers processed. With dual thermocyclers and a smart Chip Plate queue built in, you could process over 384 chambers.
All-in-one
No messing around with oils, no transferring plates, no nothing. You can add mix and samples to the chips, insert the Chip Plates and just press play. Digital PCR in an all-in-one instrument. Measuring just 56cm by 67cm, the Nio® fits in any lab.
Continuous loading
Laboratory work is not easy to schedule. Nio® allows you to add Chip Plates whenever you want, whether the instrument is running an experiment or not.