naica® system

workflow

Crystal Digital PCR™

Click on the circles to see the different steps.

PREPARE | AMPLIFY | READ | ANALYZE

The next-generation flexible Digital PCR solution.

The naica® system leverages the key assets of Digital PCR and is designed to provide a sensitive, fast, and easy-to-use solution.

DISCOVER OUR WORKFLOW

STEP 1 prepare Discover more

Only one pipetting operation for loading the reaction mixes into the wells of the microfluidic consumables.

  • OUR INNOVATIONS

naica® PCR MIX reagents

Specially formulated to ensure optimal Crystal Digital PCR™ compatibility.

Download brochure

Sapphire chip

Stilla’s pioneer digital PCR consumable intended for high sensitivity applications with the generation of up to 30 000 droplets/sample and up to 4 samples/chip

Opal chip

A higher throughput digital PCR consumable that enables the generation of up to 20000 droplets/sample and up to 16 samples/chip. The new generation chip and associated software provide a complete Crystal Digital PCR™ solution allowing desired throughput flexibility for any digital PCR application.

Download brochure
Step 2

STEP 2 amplify Discover more

Place the chips into the Geode.

Launch the Crystal Digital PCR™ program:
• Crystals of thousands of droplets are created from each sample
• PCR amplification is performed immediately after crystal generation.

Step 1
Step 3

STEP 3 read Discover more

Only one pipetting operation for loading the reaction mixes into the wells of the microfluidic consumables.

  • OUR INNOVATIONS

Prism 3

Read Sapphire and Opal chips using the 3 channels (Blue, Green and Red) of the Prism3

Prism

Introducing 6-color digital PCR

Prism offers up to 6 color target detection along with a wide range of fluorophore compatibility (FAM, ROX, Cy®5, YY® Atto 550, Cy®5.5, etc.). The high multiplexing capacity enables simultaneous absolute target quantification rendering maximum data output from precious samples. Simply place the Sapphire/Opal chips in the Prism and run the scan. The Prism scans and images the chips and renders images from each well with positive and droplets in the detection channels.

Step 2
Step 4

STEP 4 analyze Discover more

The user-friendly Crystal Miner software automatically measures the concentrations of targeted nucleic acids.

View your raw data, analyze, and explore your crystals using intuitive visuals. 

Use these free Statistical Tools to analyze your Crystal Digital PCR™ data.

Step 3

PREPARE | AMPLIFY | READ | ANALYZE

The naica® system leverages the key assets of Digital PCR and is designed to provide a sensitive, fast, and easy-to-use solution.

STEP 1 prepare

Only one pipetting action for loading the reaction mix into the wells of the chips.

  • OUR INNOVATIONS

naica® PCR MIX line

Specially formulated to ensure optimal Crystal Digital PCR™ compatibility.

Download brochure

Sapphire chip

Stilla’s pioneer digital PCR consumable intended for high sensitivity applications with the generation of up to 30 000 droplets/sample and up to 4 samples/chip

Opal chip

A higher throughput digital PCR consumable that enables the generation of up to 20000 droplets/sample and up to 16 samples/chip. The new generation chip and associated software provide a complete Crystal Digital PCR™ solution allowing desired throughput flexibility for any digital PCR application.

Download brochure

STEP 2 amplify

Place the chips into the Naica Geode
Launch the crystal Digital PCR program
• Crystals from thousands of droplets are created from each sample
• PCR amplification is performed immediately after crystal generation.

STEP 3 read

Transfer the chips to the Prism

  • Droplet crystals are imaged using up to 6 fluorescent channels
  • OUR INNOVATIONS

Prism 3

Read Sapphire and Opal chips using the 3 channels (Blue, Green and Red) of the Prism3

Prism

Introducing 6-color digital PCR

Prism offers up to 6 color target detection along with a wide range of fluorophore compatibility (FAM, ROX, Cy®5, YY® Atto 550, Atto 700...). The high multiplexing capacity enables simultaneous absolute target quantification rendering maximum data output from precious samples.

STEP 4 analyze

The user-friendly Crystal Miner software automatically measures the concentrations of targeted nucleic acids.

Analyze your data and explore your crystals using intuitive visuals.

3-color naica® system brochure
naica® PCR MIX line
Bibliography
Applications notes
Digital PCR Applications

About Crystal Digital PCR™

Crystal Digital PCR™ is Stilla’s next-generation technology for absolute quantification of nucleic acids. Using cutting-edge microfluidic innovations, this technology integrates the Digital PCR process in a single consumable.

The sample is first flowed through a network of microchannels and partitioned into a large array of individual droplets, also called a droplet crystal. PCR is then performed on-chip and the crystal is imaged to reveal the droplets that contain amplified targets. The last step consists in counting the number of these positive droplets to precisely extract the absolute quantity of nucleic acids.

With Crystal Digital PCR™, the combination of powerful image analysis and intuitive visual inspection is designed to offer an unmatched level of confidence in the digital PCR measurement, yielding reliable data.

sapphire_chip.jpeg

DIGITAL PCR IN LESS THAN 3H
3- OR 6-COLOR