Gene editing medical illustrations

Naica® System Compatible Digital PCR Test Kit and Assays

Detect and Quantify Nucleic Acids

Crystal Digital PCR™ kits allows researchers to detect and quantify nucleic acids with ready-to-use, pre-designed assays for fully-validated target panels. These digital PCR panel kits are specially developed by Stilla Technologies and its partners ID Solutions. For fully-customizable kits and/or custom assay development, contact our expert services team.

Crystal Digital PCR™ Kits for Oncology

EGFR 6-color Crystal Digital PCR™ Kit

The EGFR 6-color Crystal Digital PCR™ Kit allows for the detection and quantification of up to 32 somatic EGFR mutations in exons 18, 19, 20, and 21 associated with non-small cell cancer (NSCLC).

The EGFR 6-color Crystal Digital PCR™ Kit provides a fully validated, specific, and ultrasensitive assay for the 6-color naica® system. The kit can be used to test cell-free DNA (cfDNA) extracted from plasma samples.

The EGFR 6-color Crystal Digital PCR™ Kit is a ready-to-use solution comprised of six components, including the high-perfomance naica® multiplex PCR MIX 10X, an optimal primer and probe solution, a set of positive and negative controls, and water for sample dilution.

ReferenceProduct NameNumber of Reactions
R30006EGFR 6-color Crystal Digital PCR™ Kit48 reactions on the Sapphire Chip
EGFR 6-color Crystal Digital PCR™ Kit detection design. TaqMan® probe positions on each exon and color codes each fluorescent signal. In the table, LoB and LoD of the EGFR 6-color Crystal Digital PCR™ Kit was determined following the CLSI EP17-A2 standard (concentrations in cp/μL).

EGFR 6-color Crystal Digital PCR™ Kit technical resources

  • Application Notes

    Application Note

  • Publications

    Instructions for Use

  • Combined Shape
  • The IDENTIFY Kit Line

    The IDENTIFY digital PCR kit line provides detection for multiplexed mutation panels using fully-validated, specific, and sensitive Crystal Digital PCR™ solutions on the 3-color naica® system for a variety of oncology target panels.

    Use Crystal Digital PCR™ to test for mutations across multiple indications using circulating cell-free DNA from liquid biopsies or tumor DNA from solid biopsies. 

    The IDENTIFY Kit Line

      Microscope

      IDNRAS Solution

      Detection of NRAS mutations from tumor DNA

      Microscope

      IDEGFR Solution

      Detection of EGFR mutations in non-small cell lung cancer (NSCLC)

      Combined Shape

      IDKRAS Solution

      Detection of KRAS mutations from tumor DNA

      Microscope

      IDBRAF Solution

      Detection of BRAF mutations at position V600

      Microscope

      IDPIK3CA Solution

      Detection of PIK3CA mutations from tumor DNA

      Combined Shape

      IDIDH1-2 Solution

      Detection of IDH1 and IDH2 mutations from tumor DNA

      Microscope

      IDH3F3A Solution

      Detection of H3F3A mutations from tumor DNA

      Microscope

      IDBRAF-CNS Solution

      Detection of BRAF mutations in brain tumors

      Combined Shape

      IDFGFR1-CNS Solution

      Detection of FGFR1 mutations in brain tumors

      Combined Shape

      IDTERT Solution

      Detection of TERT gene promoter mutations in tumor DNA

    Digital PCR Kits for Covid-19

    SARS-CoV-2 Multiplex Crystal Digital PCR Kit

    The SARS-CoV-2 digital PCR test kit is intended for the detection of reported conserved viral nucleic acid sequences, Nucleoprotein N (FAM) and ORF1ab (HEX). This kit is designed to provide an advantage over conventionally-used qPCR by detecting low concentration targets in a large sample volume, where qPCR can be unreliable due to high Ct values. 

    Digital PCR test results indicate a robust and specific detection of SARS-CoV-2 sequences, down to 5 copies per 25-µl reaction equivalent to 0.2 cp/µl) for ORF1ab and for Nucleoprotein N: down to  7 copies per 25-µl reaction (equivalent to 0.28 cp/µl)). The kit includes reagents, Sapphire chips, and COVID-19 positive and negative controls.

    SARS-CoV-2 Multiplex Crystal Digital PCR Kit technical resources

    1. Webinar

      Webinar

      Learn more about how this single assay design allows for the simultaneous detection of two independent SARS-CoV-2 sequences while concurrently monitoring sample PCR effectiveness

    2. Application Notes

      Application note

    For Research Use Only. Not for use in diagnostic procedures.

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    Harness the power of the naica® system and get started with Crystal Digital PCR™ today.