Digital PCR can detect copy number of AMY1 (salivary amylase) gene which is the main cause of obesity
There is a real interest of Digital PCR in quantifying β-cell death in type 1 diabetes by detecting the level of unmethylated DNA.
To reduce the risk of graft rejection, Digital PCR can detect early transplant rejection by monitoring circulating DNA level of the transplanted organ.
Oncology is the key area of Digital PCR in which the technology is a major asset for the identification of DNA mutations (e.g EGFR), patients monitoring, gene amplification detection, CTC characterization, long non-coding RNA detection, circulating nucleic acids and tumor cells absolute quantification through liquid biopsies etc.
For health security and for higher quality product, Food industries wish to be transparent with what they produce. To do so, Digital PCR can help by detecting and quantifying GMOs, materials from GMOs or any other contaminants.
Nowadays, the number of Non-Invasive Prenatal Tests (NIPTs) are increasing, reducing the risk of miscarriage. By detecting fetal DNA in circulating blood, Digital PCR is a risk-free method for fetal genotyping: detection of aneuploidies, microdeletions, mutations and many others…
In order to increase soil fertility, organic residues, such as manure or sewage sludge, are commonly used by farmers. To assess whether human population and ecosystems are exposed to the dissemination of pathogens and antibiotic resistance genes in soil, digital PCR, allowing detection and accuracy quantification, is very useful to study those phenomena.
Digital PCR is useful for tracking viral infection and individual bacteria cells, for monitoring latent HIV reservoirs, for the measurement of residual viral load (HIV, hepatitis) etc.
Stilla’s products enable
several types of assays, allowing multiplexing :
- Absolute Quantification (DNA/ RNA)
- Rare Event Detection
- Liquid Biopsies (CTC, cfDNA)
- Whole Genome Amplification (WGA)
- NGS Library Calibration/ NGS Results Validation
- Single Cell Analysis