What are the recommended PCR Mix to use in Crystal Digital PCR™?
Depending on the application Stilla Technologies recommends the use of naica® multiplex PCR MIX for fluorescently labelled probe-based digital PCR assays or naica® PCR MIX for EvaGreen® dye-based Digital PCR assays for optimal results with Crystal Digital PCR™ for DNA amplification. These reagents are available at 5X and 10X concentrations and Stilla Technologies provides fast cycle or conventional PCR cycling protocols for initial protocol development.
More information is available here.
Stilla Technologies recommends the use of qScriptTM XLT One-Step RT-qPCR ToughMix® for RT-PCR. As an alternative the following PCR mastermixes are validated for the naica® system: PerfeCTa® MultiPlex qPCR ToughMix®, the PerfeCTa® qPCR ToughMix® UNG and PerfeCTa® qPCR ToughMix®.
Any other enzymatic mix needs to be evaluated by the user prior to performing experiments, as Stilla Technologies cannot guarantee compatibility of any other enzymatic mix with the naica® system.