Digital PCR Reagents and Chips Compatible with the Nio® and Naica® System
Digital PCR workflows do not need to be challenging. Stilla Technologies cutting-edge patented microfluidic innovation integrates the Crystal Digital PCR® workflow into a single consumable. Combined with specially-formulated Nio® and naica® PCR MIX reagents, researchers get maximized sensitivity and flexible throughput with simple reaction assembly and minimal hands-on time.
Crystal Digital PCR Chip Consumables
The next-generation Ruby Chip is an advanced Crystal Digital PCR consumable designed by scientists, for scientists—ensuring accuracy, efficiency, and reliability in every experiment.
*you can load up to 80uL of your reaction mix across multiple chambers and pool them for a higher droplet count and improved precision.
**analytical specifications are assay-dependent
Unmatched performance & precision
Flexible input volume – Load 5µL – 80µL of your sample by distributing it across up to 16 chambers.
Ultra-high resolution – 17,000 droplets/ chamber, with the flexibility to pool multiple chambers,
increasing droplet count and enhancing precision.
Smallest droplet volume – 0.22 nL, delivering the highest partition density.
Broad Dynamic Range – 5 logs for precise quantification.
Designed for all throughput needs
Scalable format – Use up to 16 samples per chip or choose to distribute the same sample across multiple chambers.
High-throughput ready – Process up to 768 samples in 24h.
Automation-compatible – Designed for streamlined lab workflows, including multichannel pipetting
and liquid handlers’ compatibility
Ready-to-use and user friendly
Single pipetting step – Thanks to pre-filled oil chambers
A unique consumable for all applications
Flexible chamber pooling – Adjust the precision and sensitivity to fit your specific needs.
Cost-Effective – Lower cost per reaction.
Ruby Chip proofs
Automation compatibility
Works with Stilla’s all-in-one digital PCR platform that harness.
Smallest reaction input volume 5 µL
You can load up to 80µL of your sample across multiple chambers and pool them for a higher droplet count and improved precision.
Smallest droplet volume (0.22 nL) & broad dynamic range (5 logs)
For the highest partition density and precise quantification.
Proven performance vs QIAcuity
Outperformed QIAcuity’s 8.5k nanoplate in sensitivity and inhibition resistance, even at 80% sample input.
Crystal Digital PCR Reagents
Specially-formulated Nio and naica PCR reagents ensure optimal performance for Crystal Digital PCR. Through Stilla’s ready-to-use reagents solutions, acquire sensitive measurements for diverse DNA and RNA applications on the Nio and naica system.
Crystal Universal Reporters
Crystal Universal Reporters generate fluorescent signals when activated during a Crystal Digital PCR reaction using target-specific Crystal Flex Probe detection chemistry.
Their universal design allows them to be used with any Crystal Digital PCR Assay, providing a modular approach to detection. This means you can use the same set of Crystal Universal Reporters to detect and quantify all targets of interest, regardless of the specific assay or the degree of multiplexing.
Select the Crystal Universal Reporter compatible with the Crystal Digital PCR® Assay of interest, either in a 200 or a 1000 Ruby Chip reaction format.
| Reference | Product Name | Volume |
|---|---|---|
| R41401 | Crystal Universal Reporter 3 | 30 µL |
| R41402 | Crystal Universal Reporter 3 | 150 µL |
| R42401 | Crystal Universal Reporter 7 | 30 µL |
| R42402 | Crystal Universal Reporter 7 | 150 µL |
Crystal Universal Reporters technical resources
naica multiplex PCR MIX (TaqMan® compatible)
The naica multiplex PCR MIX is a ready-to-use two-component solution specially formulated for optimal TaqMan fluorescent probe-based Crystal Digital PCR and high multiplexing capacity.
-The stably incorporated internal blue channel reference dye facilitates reaction assembly while ensuring reliable partition detection.
-The unique 10X concentration liberates a reaction volume that can instead be utilized by additional sample input, probes, or primers, which increases multiplexing capacity and provides superior detection sensitivity for low concentration samples and rare targets.
| Reference | Product Name | Volume |
|---|---|---|
| R10054 | naica® multiplex PCR MIX 5X | 0.750mL |
| R10055 | naica® multiplex PCR MIX 5X | 1.50mL |
| R10104 | naica® multiplex PCR MIX 10X | 0.375mL |
| R10105 | naica® multiplex PCR MIX 10X | 0.750mL |
naica multiplex PCR MIX technical resources
naica® PCR MIX (Crystal Flex Probe and EvaGreen® compatible)
The naica® PCR MIX is a ready-to-use two-component solution optimized for Crystal Flex Probe technology and EvaGreen® fluorescent intercalating dye-based Crystal Digital PCR®.
| Reference | Product Name | Volume |
|---|---|---|
| R10056 | naica® PCR MIX 5X | 1.50mL |
| R10106 | naica® PCR MIX 10X | 0.750mL |
naica PCR MIX technical resources
naica® IQ/OQ Kit
Routine Operational Qualification (OQ) procedures of the Nio®+ can be performed using the naica® IQ/OQ Kit, a ready-to-use OQ solution to closely monitor equipment compliance to operating specifications. Use the naica® IQ/OQ Kit to qualify Nio+ on a periodic basis in accordance with established standard operating procedures (SOPs).
Experience the streamlined routine OQ solution with the naica® IQ/OQ Kit for a wide range of key nucleic acid detection and quantification applications, validated for use on all Stilla Technologies dPCR platforms.
| Reference | Product Name | Number of Reactions |
|---|---|---|
| R30001 | naica® IQ/OQ Kit | 24 reactions on the Sapphire Chip (2 full OQ of the naica® system) 96 reactions on the Ruby Chip (1 full OQ of the Nio®+) |
naica® IQ/OQ Kit technical resources
Related Products
For Research Use Only. Not for use in diagnostic procedures.
Harness the power of the naica® or Nio® system and get started with Crystal Digital PCR® today.