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What hybridization temperature should I use?

If you previously ran this assay on a qPCR, you may try using the same hybridization temperature. If separability between negative and positive populations is not adequate (presence of rain, populations are close to one another), you may then optimize the assay by testing different hybridization temperatures. Do not hesitate to contact the Support Team if you need additional help at or (North America), we’ll be glad to assist you.