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Do I need a reference dye for Crystal Digital PCR™?

Yes, Fluorescein needs to be added to the PCR or RT-PCR mix for probe-based Crystal Digital PCR™ experiments. If you wish to use EvaGreen® for amplicon detection, you need to add Alexa Fluor® 647 to the mix.

For more information or queries, please contact our Support Team at or (North America)