naica® system
workflow
Click on the circles to see the different steps.
PREPARE ⬤ | AMPLIFY ⬤ | READ ⬤ | ANALYZE ⬤
The next-generation flexible Digital PCR solution.
The naica® system leverages the key assets of Digital PCR and is designed to provide a sensitive, fast, and easy-to-use solution.
Only one pipetting operation for loading the reaction mixes into the wells of the microfluidic consumables.
OUR INNOVATIONS
⬤ naica® PCR MIX line
Specially formulated to ensure optimal Crystal Digital PCR™ compatibility.
⬤ Sapphire chip
Stilla’s pioneer digital PCR consumable intended for high sensitivity applications with the generation of up to 30 000 droplets/sample and up to 4 samples/chip
⬤ Opal chip
A higher throughput digital PCR consumable that enables the generation of up to 20000 droplets/sample and up to 16 samples/chip. The new generation chip and associated software provide a complete Crystal Digital PCR™ solution allowing desired throughput flexibility for any digital PCR application.
Place the chips into the Geode.
Launch the Crystal Digital PCR™ program:
• Crystals of thousands of droplets are created from each sample
• PCR amplification is performed immediately after crystal generation.
Only one pipetting operation for loading the reaction mixes into the wells of the microfluidic consumables.
OUR INNOVATIONS
⬤Prism 3
Read Sapphire and Opal chips using the 3 channels (Blue, Green and Red) of the Prism3
⬤Prism
Introducing 6-color digital PCR
Prism offers up to 6 color target detection along with a wide range of fluorophore compatibility (FAM, ROX, Cy®5, YY® Atto 550, Cy®5.5, etc.). The high multiplexing capacity enables simultaneous absolute target quantification rendering maximum data output from precious samples. Simply place the Sapphire/Opal chips in the Prism and run the scan. The Prism scans and images the chips and renders images from each well with positive and droplets in the detection channels.
The user-friendly Crystal Miner software automatically measures the concentrations of targeted nucleic acids.
View your raw data, analyze, and explore your crystals using intuitive visuals.
Use these free Statistical Tools to analyze your Crystal Digital PCR™ data.
PREPARE ⬤ | AMPLIFY ⬤ | READ ⬤ | ANALYZE ⬤
The naica® system leverages the key assets of Digital PCR and is designed to provide a sensitive, fast, and easy-to-use solution.
STEP 1
prepare ⬤
prepare ⬤Only one pipetting action for loading the reaction mix into the wells of the chips.
OUR INNOVATIONS
⬤ naica® PCR MIX line
Specially formulated to ensure optimal Crystal Digital PCR™ compatibility.
⬤ Sapphire chip
Stilla’s pioneer digital PCR consumable intended for high sensitivity applications with the generation of up to 30 000 droplets/sample and up to 4 samples/chip
⬤ Opal chip
A higher throughput digital PCR consumable that enables the generation of up to 20000 droplets/sample and up to 16 samples/chip. The new generation chip and associated software provide a complete Crystal Digital PCR™ solution allowing desired throughput flexibility for any digital PCR application.
STEP 2 amplify ⬤
amplify ⬤Place the chips into the Naica Geode
Launch the crystal Digital PCR program
• Crystals from thousands of droplets are created from each sample
• PCR amplification is performed immediately after crystal generation.
STEP 3 read ⬤
read ⬤Transfer the chips to the Prism
- Droplet crystals are imaged using up to 6 fluorescent channels
OUR INNOVATIONS
⬤Prism 3
Read Sapphire and Opal chips using the 3 channels (Blue, Green and Red) of the Prism3
⬤ sapphire chip
Introducing 6-color digital PCR
Prism offers up to 6 color target detection along with a wide range of fluorophore compatibility (FAM, ROX, Cy®5, YY® Atto 550, Atto 700...). The high multiplexing capacity enables simultaneous absolute target quantification rendering maximum data output from precious samples.
STEP 4 analyze ⬤
analyze ⬤The user-friendly Crystal Miner software automatically measures the concentrations of targeted nucleic acids.
Analyze your data and explore your crystals using intuitive visuals.
Crystal Digital PCR™ is Stilla’s next-generation technology for absolute quantification of nucleic acids. Using cutting-edge microfluidic innovations, this technology integrates the Digital PCR process in a single consumable.
The sample is first flowed through a network of microchannels and partitioned into a large array of individual droplets, also called a droplet crystal. PCR is then performed on-chip and the crystal is imaged to reveal the droplets that contain amplified targets. The last step consists in counting the number of these positive droplets to precisely extract the absolute quantity of nucleic acids.
With Crystal Digital PCR™, the combination of powerful image analysis and intuitive visual inspection is designed to offer an unmatched level of confidence in the digital PCR measurement, yielding reliable data.
DIGITAL PCR IN LESS THAN 3H
3- OR 6-COLOR