Fluorescence spillover describes the process by which a fluorescent signal, aimed to be detected only in a given detection channel, is also detected in the adjacent detection channel. This can lead to errors in quantification, it is therefore extremely important to correct this post- acquisition by applying fluorescence spillover compensation. Please refer to the Crystal [...]
QC Flags are evaluated on three criteria: Number of analyzable droplets; Image Sharpness (whether the image is in focus or not); Number of Saturated Objects (whether some objects have a fluorescence of >65 536 RFU). There are three types of flags : Green: high quality; Green/ Yellow: moderate quality; and Yellow: Low quality. Please [...]
Visualize the droplets crystal of your chip by using the mouse scroll to zoom in the image : it should not be blurred. The flag corresponding to the sharpness should be green. If it is green and orange or orange, here is how to proceed : please verify that the chip or/and the holder [...]
We recommend that you change the focus by doing an automated focus calibration : place a chip (containing droplets with at least fluorescein in the B or C chamber) in the second position of the holder. Then log in as a manager, go in the “Settings” window and click on the “focus calibration” button. [...]
No, the focus will be set at installation of your Naica system. You do not need to set it again, unless the Prism3 has been exposed to shocks.
It is expressed in mm and it corresponds to the depth of the imaging plane used for image acquisition.
The automated estimation is performed such that you obtain the maximum separability between the positive and the negative droplet populations, while minimizing the intra-population value dispersion. You can manually adjust it (by right-cliking on the threshold) but please note that within an experiment, the threshold value will be the same for all chambers. [...]
To install the Crystal Miner software on your PC, plug the Stilla USB key and go on the "CrystalMiner Installer" directory : install first the prerequisites located in the "prerequisites" folder, then install the Crystal Miner application.
Operating system : Windows 10 in 64 bits (recommended) or Windows 7 in 64bits. RAM : at leat 16Go. Processors : at least 2cores of 2GHz or higher. Screen resolution : at least 1920x1080; aspect ratio 16:9.