What is fluorescence spillover ?

Fluorescence spillover describes the process by which a fluorescent signal, aimed to be detected only in a given detection channel, is also detected in the adjacent detection channel. This can lead to errors in quantification, it is therefore extremely important to correct this post-acquisition by applying fluorescence spillover compensation. You can refer to the [...]

2020-07-20T11:51:22+02:00July 24th, 2017|, |

How do I know if the focus value is good ?

Visualize the droplets crystal of your chip by using the mouse scroll to zoom in the image: it should not be blurred. The flag corresponding to the sharpness should be green. If it is green and orange or orange, here is how to proceed : please verify that the chip or/and the holder are [...]

2020-07-31T12:48:00+02:00July 24th, 2017|, |

How can I edit the focus value ?

We recommend that you change the focus by doing an automated focus calibration: place a chip (containing droplets with at least fluorescein in the B or C chamber) in the second position of the holder. Then log in as a manager, go in the “Settings” window and click on the “focus calibration” button. Select [...]

2020-07-31T13:28:47+02:00July 24th, 2017|, |

How is the automatic threshold calculated ?

The automated estimation is performed such that you obtain the maximum separability between the positive and the negative droplet populations while minimizing the intra-population value dispersion. You can manually adjust it (by right-clicking on the threshold). For more information or queries, please contact our Support Team at support@stilla.fr or support@stillla-inc.com (North America) [...]

2020-07-31T12:50:01+02:00July 24th, 2017|, |
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