All or some of my droplets are bigger/ smaller than what is expected. What should I do?
Make sure you are using the PCR or RT PCR recommended mix, and check that the chips are not past their expiry date.
I observe coalescences that seem to run in lines within some of my chambers
This is caused by static electricity. Please ensure that chips are not exposed to static electricity : avoid contact with hair, hands, clothes… If the problem persists, please contact support@stilla.fr, we will assess with you how to avoid this issue within your environment.
How do I get rid of an air bubble ?
If you observe an air bubble in the chamber after PCR, you may try to remove it by using the following tips: -Put the Sapphire chip back into the Geode and run the "unstacking.js" script. - If this is not sufficient, you may also try to position the air bubble next to the outlet channel, [...]
How do I avoid introducing air bubbles in the chamber?
Always use 25uL of input volume for your Crystal Digital PCR experiment. When you load the chips, make sure you do not carry any air bubbles in the pipet tip. When you prepare the PCR mixes, we recommend that you wait 2/3 minutes after the final vortex/centrifugation and before filling the chips' inlet ports. As [...]
My chamber does not contain any droplets
Make sure that the sample was loaded into the chip inlets. Check that the Naica Geode is connected to the pressure source and that the pressure profiles are similar to the ones described in the Naica System user manual. Check that the appropriate amount of fluorescein (100nM) was included in the reaction mix.