When performing a Crystal Digital PCR experiment, you only need the Sapphire chips and PCR caps. Everything you need is conveniently contained within the Sapphire chips box delivered to you.
Can I use only a subset of chambers of a Sapphire chip for a run, then use the empty chambers of the same chip for another run?
We do not recommend such usage, mostly due to concerns over potential contamination. Indeed, we do not advise that any consumable that has been in a post-PCR environnment is returned to a prePCR lab.
Yes, you may perform a run overnight and read it the next day. This will not affect your results.
Sapphire chips are stored at room temperature (15-25°C).
Based on our results obtained with the PerfeCTa mix, the droplet size variation is 5% (droplet diameter).
You may request additional caps for the Sapphire chips at email@example.com or firstname.lastname@example.org.
At this time, we only offer Sapphire chips. Please note that we are currently developping an alternative consumable which allows the analysis of up to 48 samples / run.
Using the Sapphire chips, you may quantify from 0.2 copies/ uL to 20,000 copies/ uL.
The total number of droplets analyzed is between 15,000-30,000 per chamber.
The droplets generated have a mean volume of ~0.59 nL when validated using Perfecta® qPCR ToughMix™, qPCR ToughMix® UNG, Multiplex qPCR ToughMix® for PCR and a mean volume of ~ 0.55 nL when validated using qScript™ XLT One-Step RT-qPCR ToughMix® for RT-PCR.