No. Stilla only provides the instruments, the chips, the analysis software… and the expertise! Please contact your local distributor for any of the PCR reagents needed for Crystal Digital PCR.
You may use any fluorophore you wish with the Naica system, provided that their spectra fall within the excitation/ emission spectra of the Prism3. Blue channel: Em 415-480 nm/ Ex 495-520 nm ; Green: Ex 530-550 nm/ Em 560-610 nm; Red: Ex 615-645 nm/ Em 655-720 nm.
You may use EvaGreen®, please refer to the "Crystal Digital PCR™ Using EvaGreen" Application Note available here https://www.stillatechnologies.com/crystal-digital-pcr-using-evagreen/ for detailed instructions. Please make sure to use the correct analysis configuration file associated with this protocol. For more information or queries, please contact our Support Team at support@stilla.fr or support@stillla-inc.com (North America)
No, SYBR® Green use is not compatible with the Sapphire chips. Please use EvaGreen® instead. For more information or queries, please contact our Support Team at support@stilla.fr or support@stillla-inc.com (North America)
We recommend using 1 uM final concentration for primers as a starting concentration for probe-based detection and 200 nM for EvaGreen-based detection.
We recommend using 250 nM final concentration for probes.
Our assays are calibrated with the PerfeCTa PCR mixes (Quanta Bio), hence we highly recommend their use. Please note that except for the PerfeCTa PCR mixes, we have not perform extensive calibration so we won't be able to provide you information about the size of the droplets, reproducibility...
Yes, you can perform one-step RT-PCR in droplets using the Quanta Bio qScript XLT One Step RT-qPCR Toughmix (no ROX). Please remember that you need to add fluorescein to the reaction.
Our assays are calibrated with the PerfeCTa PCR mixes (Quanta Bio), hence we highly recommend their use. Please note that except for the PerfeCTa PCR mixes, we have not perform extensive calibration so we won't be able to provide you information about the size of the droplets, reproducibility...
Our Crystal Digital PCR process is compatible with Quanta Bio PerfeCTa Multiplex qPCR Toughmix (No ROX), PerfeCTa qPCR Toughmix (no ROX) and PerfeCTa qPCR Toughmix UNG (no ROX). Please visit the Quanta Bio website (http://www.quantabio.com) to find distributors in your country.