Yes, Stilla® recently launched its brand new range of naica® PCR mastermixes that are optimized for high performing Crystal Digital PCR™ assays. For more details about the PCR mastermixes, please visit: naica® line of PCR Mixes compatible with the naica® system
You may use any fluorophore you wish with the naica® system, provided that their spectra fall within the excitation/ emission spectra of the Prism3. Blue channel: Em 415-480 nm/ Ex 495-520 nm ; Green: Ex 530-550 nm/ Em 560-610 nm; Red: Ex 615-645 nm/ Em 655-720 nm.
You may use EvaGreen®, please refer to the "Crystal Digital PCR™ Using EvaGreen" Application Note available here https://www.stillatechnologies.com/crystal-digital-pcr-using-evagreen/ for detailed instructions. Please make sure to use the correct analysis configuration file associated with this protocol. For more information or queries, please contact our Support Team at support@stilla.fr or support@stillla-inc.com (North America)
No, SYBR® Green use is not compatible with the Sapphire chips. Please use EvaGreen® instead. For more information or queries, please contact our Support Team at support@stilla.fr or support@stillla-inc.com (North America)
Recommended primer and probes concentrations on the naica® system are 0.25 to 1 µM.
We recommend using 250 nM final concentration for probes.
Our assays are calibrated with the PerfeCTa PCR mixes (Quanta Bio), hence we highly recommend their use. Please note that except for the PerfeCTa PCR mixes, we have not perform extensive calibration so we won't be able to provide you information about the size of the droplets, reproducibility...
Yes, you can perform one-step RT-PCR in droplets using the Quanta Bio qScript XLT One Step RT-qPCR Toughmix (no ROX). Please remember that you need to add fluorescein to the reaction.
Depending on the application Stilla Technologies recommends the use of naica® multiplex PCR MIX for fluorescently labelled probe-based digital PCR assays or naica® PCR MIX for EvaGreen® dye-based Digital PCR assays for optimal results with Crystal Digital PCR™ for DNA amplification. These reagents are available at 5X and 10X concentrations and Stilla Technologies provides [...]
Depending on the application Stilla Technologies recommends the use of naica® multiplex PCR MIX for fluorescently labelled probe-based digital PCR assays or naica® PCR MIX for EvaGreen® dye-based Digital PCR assays for optimal results with Crystal Digital PCR™ for DNA amplification. These reagents are available at 5X and 10X concentrations and Stilla Technologies provides [...]