What are the compatible fluorophores?

You may use any fluorophore you wish with the naica® system, provided that their spectra fall within the excitation/ emission spectra of the Prism3. Blue channel: Em 415-480 nm/ Ex 495-520 nm ; Green: Ex 530-550 nm/ Em 560-610 nm; Red: Ex 615-645 nm/ Em 655-720 nm.

2020-11-06T18:10:39+01:00July 24th, 2017|, |

I want to perform experiment using intercalating dye?

You may use EvaGreen®, please refer to the "Crystal Digital PCR™ Using EvaGreen" Application Note available here https://www.stillatechnologies.com/crystal-digital-pcr-using-evagreen/ for detailed instructions. Please make sure to use the correct analysis configuration file associated with this protocol. For more information or queries, please contact our Support Team at support@stilla.fr or support@stillla-inc.com (North America)

2020-07-31T12:52:21+02:00July 24th, 2017|, |

Can I use PCR Mix other than the Quanta Bio mixes recommended by Stilla?

Depending on the application Stilla Technologies recommends the use of naica® multiplex PCR MIX for fluorescently labelled probe-based digital PCR assays or naica® PCR MIX for EvaGreen® dye-based Digital PCR assays for optimal results with Crystal Digital PCR™ for DNA amplification. These reagents are available at 5X and 10X concentrations and Stilla Technologies provides [...]

2021-01-08T08:14:38+01:00July 24th, 2017|, |

What are the recommended PCR Mix to use in Crystal Digital PCR™?

Depending on the application Stilla Technologies recommends the use of naica® multiplex PCR MIX for fluorescently labelled probe-based digital PCR assays or naica® PCR MIX for EvaGreen® dye-based Digital PCR assays for optimal results with Crystal Digital PCR™ for DNA amplification. These reagents are available at 5X and 10X concentrations and Stilla Technologies provides [...]

2021-01-08T08:17:17+01:00July 24th, 2017|, |
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