Our unique technology, Crystal Digital PCR™, relies on sample partitioning into arrays of droplets, which are assembled into each of the rectangular wells of the Sapphire chip. Within this well, we can observe that the droplets self-assemble into a periodic arrangement reminiscent of the pattern found for atoms within crystalline structures. This is why [...]
You may refer to our naica® system user manual (can be found here) for guidelines, a whole host of descriptive items, and step-by-step tutorials on how to set up digital PCR experiments on gene-pi.com, an educational website equipped with resources and information about digital PCR. We would also like to refer you to the [...]
In real-time PCR, quantification is achieved using a standard curve or is expressed relatively to a reference sample. In digital PCR, you can obtain absolute quantification without the need for references. Moreover, when attempting to detect or quantify rare events in a complex sample, such as a rare mutant in a background of wild-type [...]
In real-time PCR, fluorescence intensity is measured at each cycle, and is compared to the fluorescence pattern measured for standards or references for quantification. This is thus an analog measurement. Digital PCR on the other hand will measure discrete values, the number of positive partitions and the number of total partitions, leading to absolute [...]
Digital PCR is a novel implementation of PCR that relies on dividing the initial reaction mix into multitude of smaller reactions. In Crystal Digital PCR™, this is achieved through dividing the reaction mix into tens of thousands of subnanoliter droplets. DNA molecules thus partitioned into droplets are amplified separately, and a fluorescent reporter, such [...]
Yes, you may image the chips prior to amplification, please refer to the "Imaging Droplets Crystal Prior to PCR" protocol for instructions available here: https://www.stillatechnologies.com/technical-resources/(secured access) or contact the support team at firstname.lastname@example.org or email@example.com (North America).
Yes, you can recover droplets after PCR. To request the protocol, please contact firstname.lastname@example.org or email@example.com (North America). You can also read the Application Note on "Droplet Recovery" here https://www.stillatechnologies.com/droplet-recovery-with-crystal-digital-tm-pcr/
If you previously ran this assay on a qPCR, you may try using the same hybridization temperature. If separability between negative and positive populations is not adequate (presence of rain, populations are close to one another), you may then optimize the assay by testing different hybridization temperatures. Do not hesitate to contact the Support [...]