What is a droplet crystal?

Our unique technology, Crystal Digital PCR, relies on sample partitioning into 2D monolayer arrays of droplets, which are assembled into each of the rectangular chambers of the Sapphire chip. Within this chamber, we can observe that the droplets self-order into a periodic arrangement reminiscent of the pattern found for atoms within crystalline structures. This [...]

2017-09-07T14:02:34+02:00August 1st, 2017|, |

What are the advantages of digital PCR versus real-time PCR?

In real-time PCR, quantification is achieved using a standard curve or is expressed relatively to a reference sample. In digital PCR, you can obtain absolute quantification without the need for references. Moreover, when attempting to detect or quantify rare events in a complex sample, such as a rare mutant in a background of wild-type [...]

2020-07-20T11:55:25+02:00August 1st, 2017|, |

What is the difference between real-time PCR and digital PCR?

In real-time PCR, fluorescence intensity is measured at each cycle, and is compared to the fluorescence pattern measured for standards or references for quantification. This is thus an analog measurement. Digital PCR on the other hand will mesure discrete values, the number of positive partitions and the number of total partitions, leading to absolute [...]

2017-09-07T13:53:21+02:00August 1st, 2017|, |

What is digital PCR?

Digital PCR is a novel implementation of PCR that relies on dividing the initial reaction mix into multitude of smaller reactions. In Crystal Digital PCR, this is achieved through dividing the reaction mix into tens of thousands of subnanoliter droplets. DNA molecules thus partitioned into droplets are amplified separately, and a fluorescent reporter, such [...]

2020-07-20T11:58:20+02:00August 1st, 2017|, |

What hybridization temperature should I use?

If you previously ran this assay on a qPCR, you may try using the same hybridization temperature. If separability between negative and positive populations is not adequate (presence of rain, populations are close to one another), you may then optimize the assay by testing different hybridization temperatures. Do not hesitate to contact the Support [...]

2020-07-30T16:35:05+02:00July 24th, 2017|, |
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