What is fluorescence spillover ?

Fluorescence spillover describes the process by which a fluorescent signal, aimed to be detected only in a given detection channel, is also detected in the adjacent detection channel. This can lead to errors in quantification, it is therefore extremely important to correct this post-acquisition by applying fluorescence spillover compensation. You can refer to the Crystal Miner user manual available here for detailed instructions on compensation and read this item on gene-pi.com for a detailed description and understanding of fluorescence spillover compensation.