QC Flags are evaluated on three criteria: Number of analyzable droplets; Image Sharpness (whether the image is in focus or not); Number of Saturated Objects (whether some objects have a fluorescence of >65 536 RFU). There are three types of flags : Green: high quality; Green/ Yellow: moderate quality; and Yellow: Low quality. Please note that the QC flag for each sample will always take the worst individual flag status. Similarly for the experiment: if one of the chambers has got a yellow flag, the experiment as a whole is automatically assigned a yellow flag.