What are the recommended PCR mixes to use in Crystal Digital PCR®?

Depending on the application, Stilla Technologies recommends the use of naica® multiplex PCR MIX for fluorescently labelled probe-based digital PCR assays or naica® PCR MIX for EvaGreen® dye-based digital PCR assays for optimal results with Crystal Digital PCR® for DNA amplification. These reagents are available at 5X and 10X concentrations and Stilla Technologies provides PCR cycling protocols for initial protocol development.

More information is available here.

Stilla Technologies recommends the use of qScript^TM XLT One-Step RT-qPCR ToughMix® for RT-PCR.