Ensure that the pressure source is activated and adjust the pressure delivered to the Geode to 1250 +/- 50 mbar.
When the measured pressure is higher than 300 mbars, a pneumatic lock prevents the lock to be opened for security purposes. In all cases, you should wait for the current pressure to be lower than 20mbars and the current temperature to be lower than 40C before opening the lid. If the waiting time is longer [...]
Press the top blue button located on the rear side of the Geode. If the screen does not respond, reboot the Geode : switch off the back power button, wait a few seconds and switch on the back power button.
Depending on the time power has been out, choose to restart or abort the experiment. Please note that if the pressure within the Geode has decreased below 850 mBar and/ or the temperature of the hot plate has decreased to room temperature, you may not be able to obtain results from your experiment.
In order to avoid overheating of the Geode, could you please verify that these conditions are satisfied : a minimum distance of 25cm to neighbouring objects around the Geode a room temperature below 25°C
In order to refine the diagnostic, we will need logs files automatically generated by the Geode. Here is the procedure to follow to recover those files : Start the Geode (if it is not already done) Plug a USB stick in front of the Geode, wait for its recognition and then select “validate” (green icon) [...]
The image analysis algorithm of Crystal Miner discriminates between true droplets and artefacts based on the following criteria: Size : a true droplet should have a minimum diameter and maximum diameter Shape : a true droplet should have a minimum circularity and a maximum eccentricity Texture : a true droplet should have a texture that [...]
Some droplets in the crystal overlap and are not recognized by the software. How do I solve this issue?
You may place the chips back in the Geode without removing any caps. Then start the "unstacking.js" program. You may now scan your chips again. If this program proved unsufficient, you may play the program again until you get a satisfactory result.
If you observe an air bubble in the chamber after PCR, you may try to remove it by using the following tips: -Put the Sapphire chip back into the Geode and run the "unstacking.js" script. - If this is not sufficient, you may also try to position the air bubble next to the outlet channel, [...]
Always use 25uL of input volume for your Crystal Digital PCR experiment. When you load the chips, make sure you do not carry any air bubbles in the pipet tip. When you prepare the PCR mixes, we recommend that you wait 2/3 minutes after the final vortex/centrifugation and before filling the chips' inlet ports. As [...]