Recovering droplets from Sapphire Chips
The Naica™ System allows the recovery of droplets from the Sapphire chips with a simple procedure that can be performed before or after PCR. DNA contained in the recovered emulsion is extracted using a standard chloroform protocol and is suitable for downstream analysis using NGS, digital or real-time PCR, or gel electrophoresis. The recovery and extraction procedure takes approximately 1h for up to 24 samples (Fig. 1).
DNA Recovery Rate
The recovery rate for this protocol was assessed by generating a droplet crystal containing known amounts of human genomic DNA, counting droplets in the Sapphire Chips before and after recovery, extracting DNA from droplets using the protocol described above, and quantifying the amount of DNA recovered by Crystal Digital PCR. We found that:
• at least 98% of the droplets are recovered from the Sapphire Chips
• on average 70% of the total DNA present in the initial droplet crystal is recovered (Fig. 2)