How To Setup Analyze and Report for PCR Primer Design

Digital PCR Tutorials

Performing Digital PCR Hands-On

Each digital PCR platform has its own hands-on protocol. In this item, we have chosen to describe protocols used on…

Copy Number Variation Calculation for your PCR Experiment

Copy Number Variation (CNV) Copy Number Variation (CNV) detection is a critical tool for estimating the variation in the copy…

MIQE Guidelines

How to report In order to ensure experimental transparency, consistency between research laboratories and thus, maintain a high level of…

Poisson Law Computation

Poisson distribution Poisson distribution, named after the French mathematician Siméon Denis Poisson, is the probability of occurrence of a given number of…

Dynamic Ranges of Detection & Quantification

Calculation First of all, let’s assume that a target gene concentration has been estimated together with its relative uncertainty, by…

Advanced Threshold Setting

Advanced Threshold Setting When the different populations are not well separated, automatic thresholding fails and must be manually adjusted. The threshold…

DNA Preparation for Digital PCR

Digital PCR DNA Preparation DNA preparation is a crucial step for the success of your digital PCR assay. Template extraction,…

Digital PCR Assay Optimization

Sub-optimal digital PCR settings Sub-optimal digital PCR settings very often lead to insufficient signal to noise ratio. This affects the…

Designing Primers & Probes

Rules Primer and probe design is a crucial step for a successful experiment. The rules for designing primers and probes…